L-Alanine Dehydrogenase from Bacillus subtilis

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一般描述

L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain.

应用

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .
L-Alanine Dehydrogenase from Bacillus subtilis has been used in the carbon nanotube columns for H2-driven biocatalysis hydrogenation studies.

包装

250, 500 units in glass bottle

生化/生理作用

L-Alanine Dehydrogenase is essential for sporulation in Bacillus subtilis.
L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

单位定义

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH₃ per min at pH 10.0 at 25 ℃.

外形

Suspension in 2.4 M (NH₄)₂SO₄ solution, pH 7.0

基本信息

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安全信息

象形图
警示用语：	Danger
危险声明	H334
预防措施声明	P261 - P284 - P304 + P340 + P312 - P501
危险分类	Resp. Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)