Avidin–Peroxidase

半岛bd体育手机客户端 说明

一般描述

Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

应用

Avidin-Peroxidase has been used for ELISA applications. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

制备说明

Affinity purified protein.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00130587
NACRES	NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	avidin from egg white enzyme from horseradish
偶联物	peroxidase conjugate
形式	buffered aqueous solution
technique(s)	indirect ELISA: 1:100,000 western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable