半岛bd体育手机客户端 说明

一般描述

The Na⁺/K⁺ATPase (NKA) enzyme contains α, β and γ subunits. This enzyme is widely expressed. ATPase Na⁺/K⁺transporting subunit α 1 (ATP1A1) is a principal catalytic subunit of NKA. It has a molecular mass of about 110kDa with 10 transmembrane segments. α-subunit has four different isoforms with tissue specific expression. α1 is primarily expressed in various tissues, including kidney, nerves and lung. While α2 is expressed in skeletal muscle and heart, α3 is found in the brain and α4 is apparently localized to testis and specifically to spermatozoa. ATP1A1 is encoded by the gene mapped to human chromosome 1p13-p11.

免疫原

sheep kidney α subunit of Na⁺/K⁺ATPase.

应用

Monoclonal Anti- Na⁺/K⁺ATPase ( α Subunit) antibody produced in mouse has been used in flow cytometry.

生化/生理作用

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the α subunit, and an associated smaller glycoprotein, termed the β subunit. Studies indicate that there are three isoforms of the α subunit (α 1, α 2, α 3) and two isoforms of the β subunit (β 1 and β 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The α 1 and β mRNAs are present in all cell types examined, whereas the α 2 and α 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The α subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.
The Na⁺/K⁺ATPase is implicated in the maintenance of ion homeostasis for regular neuronal excitability, secondary transport and neurotransmitter uptake. ATPase Na⁺/K⁺transporting subunit α 1 (ATP1A1) participates in both pumping and signaling functions. ATP1A1 regulates cellular transmembrane sodium and potassium ion gradients essential for the control of resting membrane potential. Additionally, nucleotide binding domain of ATP1A1 facilitates cellular signal transduction and cell growth. Deficiency of the gene leads to development of major depressive disorder (MDD).

目标描述

Na⁺/K⁺transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

外形

solution containing 1 mg/ml BSA and 0.05% sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02096998
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	M7-PB-E9, monoclonal
分子量	antigen ~110 kDa
species reactivity	pig, canine, bovine, sheep, chicken, human, mouse
should not react with	rat,Xenopus
technique(s)	immunocytochemistry: suitable immunofluorescence: 1:20 immunohistochemistry (frozen sections): 1:100 immunoprecipitation (IP): suitable indirect ELISA: suitable western blot: 1:500-1:5000
同位素/亚型	IgG1
UniProt登记号	P05023
运输	dry ice
储存温度	?20℃
Gene Information	human ... ATP1A1(476)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)