Anti-SIRT2 Antibody

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一般描述

Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.

特异性

This antibody recognizes the mature protein of SIRT2.

免疫原

Epitope: Mature protein
KLH-conjugated linear peptide corresponding to the mature protein of human SIRT2.

应用

Use Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Western Blot (SNAP ID) Analysis: 0.25 ?g/mL from a representative lot detected SIRT2 on 10 ?g of human brain tissue lysate.

Immunoprecipitation Analysis: 5 ?g from a representative lot immunoprecipitated SIRT2 in human brain lysate.

质量

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.125 ?g/mL of this antibody detected SIRT2 on 10 ?g of human brain tissue lysate.

目标描述

Observed molecular weight is ~39 kDa Multiple isoforms around 40 kDa may be observed.

联系

Replaces: 04-1124

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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