Anti-phospho-EGFR (Tyr1069) Antibody

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一般描述

EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon ligand binding, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream. Tyr1069 is phosphorylated during activation.

特异性

Reacts strongly and specifically with Tyr1069 phosphorylated EGFR

免疫原

Tyrosine-phosphorylated epidermal growth factor receptor.
Epitope: Tyr1069 (cytoplasmic domain)

应用

Immunofluroescent Immunocytochemistry:
This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069).
Research Category
Apoptosis & Cancer

Signaling
Research Sub Category
Kinases & Phosphatases

质量

Routinely evaluated by Western Blot on untreated and EGF treated A431 lysates.

Western Blot Analysis:
1:1,000 dilution of this antibody detected Tyr1069 phosphorylated EGFR on 10 ?g of EGF treated A431 lysates.

目标描述

~170 kDa

联系

Replaces: 04-339

外形

Antigen affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl, with 0.05% sodium azide.
Antigen Affinity Purified

储存及稳定性

Stable for 1 year at 2-8?C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

分析说明

Control
Untreated and EGF treated A431 lysates

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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