Anti-acetyl Cortactin Antibody

半岛bd体育手机客户端 说明

一般描述

Cortactin is a cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.

Recent research efforts show cortactin is acetylated in vivo and is deacetylated by HDAC6 (Histone deacetylase 6). HDAC6 is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In recent studies it was demonstrated that HDAC6 binds cortactin and alters the ability of cortactin to bind F-actin by modulating a “charge patch” in its repeat region. Findings suggest that, in addition to its role in microtubule-dependent cell motility, HDAC6 influences actin-dependent cell motility by altering the acetylation status of cortactin, which, in turn, changes the F-actin binding activity of cortactin. The acetylation level of cortactin may correlate to tumor metastases and acetylated cortactin may be considered a potential metastasis marker.

特异性

The antibody recognizes human acetylated Cortactin.

免疫原

Epitope: Unknown
Consensus sequence motif of acetylation sites of cortactin.

应用

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-acetyl Cortactin Antibody is validated for use in Western Blotting, for the detection of acetyl Cortactin.

质量

Evaluated by Western Blot in HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.

Western Blot Analysis: 2.5 ?g/ml of this antibody detected Cortactin on 10 ?L of HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.

目标描述

~ 85 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse
species reactivity (predicted by homology)	Drosophila(based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)	western blot: suitable
NCBI登记号	NP_005222.2
UniProt登记号	Q14247
运输	wet ice
Gene Information	human ... CTTN(2017)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable