您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Cas9 Hygromycin Lentiviral Particles

Cas9 Hygromycin Lentiviral Particles

品牌
Sigma-Aldrich
货号
CAS9HYGROV
包装型号
规格纯度
价格
3752.73 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Cas9 Hygromycin Lentiviral Particles
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Ready-to-use Cas9-Hygro lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-Hygro lentiviral particles efficiently and stably integrate Cas9 and a hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-Hygro) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.

To order gRNA in any format click here

应用

Functional Genomics/Target Validation

  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Creation of cell lines stably expressing Cas9

特点和优势

  • Highly specific and highly active
  • Sequence verified
  • Ready to infect/transduce

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacteriumStreptococcus pyogeneshas been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

单位定义

VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.

法律信息

CRISPR Label License

Lentiviral, WPRE, and Evrogen Licenses

半岛bd体育手机客户端 性质

包装 vial of 200 μL
浓度 ≥1 x 106VP/ml virus (via p24 assay)
application(s) CRISPR
selection blasticidin
运输 dry ice
储存温度 ?70℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map