Cas9 Plus Protein

半岛bd体育手机客户端 说明

一般描述

Recombinant Cas9 Plus protein fromStreptococcus pyogenes(~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs,Streptococcus pyogenesCas9 Plus protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

应用

Functional Genomics; Target Validation; Genome Editing

特点和优势

• Enhanced specificity compared to wild type Cas9
• Increased activity compared to enhanced specificity Cas9
• Ready to inject/transfect

包装

pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)

组分

Each kit consists of:

• one vial of Cas9 Plus Recombinant Protein
• one vial of Plus Transfection Buffer
• one vial of Reconstitution Buffer
• one vial of Dilution Buffer

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacteriumStreptococcus pyogeneshas been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

重悬

LyophilizedS. pyogenesCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.

其他说明

Use our CRISPR Selection Tool to order gRNA

Check out our other MISSION^?Cas9 Proteins at SigmaAldrich.com/CRISPRproteins

法律信息

CRISPR Use License Agreement
MISSION is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli
测定	≥95% (SDS-PAGE)
形式	lyophilized powder
包装	pkg of 1 kit (4 components)
application(s)	CRISPR
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 3