CD44 (MRQ-13) Mouse Monoclonal Antibody

半岛bd体育手机客户端 说明

一般描述

The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kD or hematopoietic variant (CD44s) that is found in mesodermal cells such as hematopoietic, fibroblastic, and glial cells, and in some carcinoma cell lines. Higher molecular weight isoforms have been described in epithelial cells (CD44v) and are thought to function in intercellular adhesion and stromal binding. While the other functions and distributions of the CD44 family have not yet been completely elucidated they are also known to participate in embryonic development and angiogenesis as well as other molecular processes associated with specific adhesions, signal transduction, and cell migration. The recent demonstration of a concordance of the cell proliferation nuclear antigen, Ki-67, and CD44 expression in adenomatous polyps, colonic carcinomas and adjacent mucosa raises the possibility of involvement of CD44 in stimulating cell growth. It appears that the CD44-hyaluronate interaction is central to tumor invasiveness; the receptor allowing the uptake and subsequent degradation of metrical hyaluronate. While many human tumors express CD44, a positive correlation between increased CD44v expression and tumor progression and/or dedifferentiation has been demonstrated in only some. Such tumors include non-Hodgkin′s lymphoma (Stauder et al, 1995), hepatocellular carcinoma (Matthew et al, 1996), breast carcinoma, renal cell carcinoma (terpe et al, 1993), colonic carcinoma (Abassi et al, 1993; Wielenga et al, 1993; Herrlich et al, 1995) and some soft tissue tumors (Wand et al, 1996). More recent additions to the list include metastatic melanoma (Sviatoha, et al, 2002), prostatic carcinoma (Ekici et al, 2002), and gastric cancer (Yamaguchi et al, 2002). Conversely, CD44v expression is downgraded in other tumors including neuroblastoma (Shtivelman & Bisho, 1991), squamous cell and basal cell carcinomas of the skin (Herold-Mende et al, 1996). The suggestion that there is a positive association between CD44 isoform expression and progression in human tumors has important implications for diagnosis and prognosis. Unfortunately, the situation is not yet clear-cut. Confusion over the complicated exon boundaries together with the different nomenclature employed by researchers have added to problems of identifying the true metastasis-associated isoforms. Furthermore, stromal cells may contribute to the isoform pattern detected. Probably the most practical application of CD44 immunostaining at present is the discrimination of urothelial transitional call carcinoma-in-situ from non-neoplastic changes in the urothelium (McKenney JK, et al. 2001).

质量

IVD

IVD

IVD

RUO

联系

CD44 Positive Control Slides, Product No. 144S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

外形

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

制备说明

Download the IFU specific to your product lot and format
Note: This requires a keycode which can be found on your packaging or product label.

Download the latest released IFU
Note: This IFU may not apply to your specific product lot.

其他说明

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

法律信息

Cell Marque is a trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	500 100
生物来源	mouse
偶联物	unconjugated
抗体形式	culture supernatant
antibody product type	primary antibodies
克隆	MRQ-13, monoclonal
描述	For In Vitro Diagnostic Use in Select Regions (See Chart)
形式	buffered aqueous solution
species reactivity	human
包装	vial of 0.1 mL concentrate (144M-94) vial of 0.5 mL concentrate (144M-95) bottle of 1.0 mL predilute (144M-97) vial of 1.0 mL concentrate (144M-96) bottle of 7.0 mL predilute (144M-98)
manufacturer/tradename	Cell Marque?
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
同位素/亚型	IgG2a
控制	benign urothelium
运输	wet ice
储存温度	2-8℃
可视化	membranous