Anti-trimethyl-Histone H3 (Lys9) Antibody

半岛bd体育手机客户端 说明

一般描述

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

特异性

A broad species cross-reactivity is expected.
Recognizes Histone H3 containing trimethyl-lysine 9 and, to a lesser extent, dimethyl-lysine 9. MW ~17 kDa.

免疫原

Epitope: Trimethyl Lys9
BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl-lysine 9 of human Histone H3.

应用

Anti-trimethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9) & has been validated in DB, PIA & WB.
Peptide Inhibition Analysis:
10 μM of a Histone H3 peptide containing trimethyl-lysine 9 abolished detection of Histone H3 by anti-trimethyl Histone H3 (Lys9) in western blot analysis of HeLa acid extracts (Figure B, lane 4). Slight signal reduction was observed with 10 μM of a Histone H3 peptide containing dimethyl-lysine 9.

Peptide Dot Blot Analysis:
A 1:2000 dilution of a previous lot was incubated with serial dilutions of Histone H3 peptides containing various modifications spotted onto PVDF. Slight cross-reactivity with Histone H3 peptide containing dimethyl-lysine 9 was detected.

Beadlyte Histone Peptide Assay:
1:2000-1:6000 dilutions of a previous lot were incubated with Histone H3 peptides containing various modifications conjugated to Luminex microspheres. Slight cross-reactivity with Histone H3 peptides containing dimethyl-lysine 9 and trimethyl-lysine 27 was detected.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by western blot in acid-extracted proteins from HeLa cells.

Western Blot Analysis:
1:500-1:5000 dilution of this antibody detected methylated Histone H3 (Lys9) in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).

目标描述

~17 kDa

外形

Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide before the addition of 30% glycerol.
Immunodepleted Serum

储存及稳定性

Stable for 1 year at -20?C from date of receipt.

分析说明

Control
Acid extracted proteins from HeLa cells.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human
manufacturer/tradename	Upstate?
technique(s)	dot blot: suitable inhibition assay: suitable (peptide) western blot: suitable
同位素/亚型	IgG
NCBI登记号	NP_003484
UniProt登记号	Q16695
运输	dry ice
Gene Information	human ... H3F3B(3021)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable