Anti-phospho-SRPK2 (Ser494) Antibody

半岛bd体育手机客户端 说明

一般描述

Along with Serine/threonine protein kinase 2 (SRPK2), SRPK1 phosphorylates serine residues found in RS-domain-containing proteins, such as SFRS1 and SFRS2. These kinases also phosphorylate the same serine residues on the HBV core protein both in vitro and in vivo. The cytoplasmic proteins are critical for nuclear import of SR proteins in a phosphorylation-dependent manner. Additionally, they mediate the trafficking of splicing factors and play a role in spliceosome assembly and mitogenesis.

特异性

This antibody is specific for the protein kinase domain of SRPK2 phosphorylated at Ser494, independent of observed phosphorylation at Ser497.

免疫原

KLH-conjugated linear peptide corresponding to the protein kinase domain of SRPK2 phosphorylated at Ser494.
Epitope: Protein kinase domain

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Peptide Inhibition Analysis: 1 ?g/mL from a representative lot detected SRPK2 in 10 ?g of insulin treated HepG2 cell lysate.
Research Category
Signaling
Anti-phospho-SRPK2 (Ser494) Antibody is an antibody against phospho-SRPK2 (Ser494) for use in WB, PIA.

质量

Evaluated by Western Blot in untreated and insulin treated HepG2 cell lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected SRPK2 in 10 ?g HepG2 cell lysate.

目标描述

~120 kDa observed. Two isoforms at ~77 kDa and ~79 kDa may be observed in some cell lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Untreated and insulin treated HepG2 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse
species reactivity (predicted by homology)	rat (based on 100% sequence homology),Xenopus(based on 100% sequence homology)
technique(s)	inhibition assay: suitable (peptide) western blot: suitable
NCBI登记号	NP_872633
UniProt登记号	P78362
运输	wet ice
Gene Information	human ... SRPK2(6733)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable