Anti-NR2A Antibody

半岛bd体育手机客户端 说明

一般描述

NR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

特异性

Recognizes NR2A, MW ~170 kDa.

免疫原

6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A).

应用

Detect NR2A using this Anti-NR2A Antibody validated for use in IP & WB.
Immunoprecipitation:
4 ?g of a previous lot immuno-precipitated NR2A from rat brain hippocampus membranes and rat brain microsomal preparations.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

质量

Evaluated by western blot in rat brain microsomal preparations (Catalog # 12-144).

Western Blot Analysis:
0.5-2 ?g/mL of this antibody detected NR2A in rat brain microsomal preparations (Catalog # 12-144).

目标描述

~170 kDa

外形

Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

储存及稳定性

Stable for 1 year at -20?C from date of receipt.

分析说明

Control
Brain tissue.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	rat, mouse
manufacturer/tradename	Upstate?
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG
NCBI登记号	NM_008170
UniProt登记号	Q12879
运输	wet ice
Gene Information	human ... GRIN2A(2903)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable