半岛bd体育手机客户端 说明
一般描述
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that remove specific methyl marks from histones. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
JMJD4 is a transcription factor that is a putative histone demethylase. It is highly expressed in lymphoid (spleen) and hemopoietic (bone marrow) tissues
特异性
This antibody recognizes the N-terminal region of human JMJD4.
免疫原
Epitope: N-terminus
The immunogen was a KLH-conjugated synthetic peptide corresponding to the N-terminal region of human JMJD4.
应用
Research Sub Category
Histone Modifying Proteins
Chromatin Biology
Use Anti-JMJD4 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, ELISA to detect JMJD4 (N-terminus) also known as Jumonji domain-containing protein 4.
Research Category
Epigenetics & Nuclear Function
ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
HeLa and NIH/3T3 cells incubated with anti-JMJD4 (N-terminus) stained positively on nuclei.
质量
Western Blot Analysis:
1:250 dilution of this antibody detected JMJD4 on 10 ?g of A431 lysate
目标描述
~53 kDa
外形
Protein G Chromatography
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Format: Purified
储存及稳定性
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20?C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
A431 lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | mouse, human |
| technique(s) | ELISA: suitable immunocytochemistry: suitable western blot: suitable |
| NCBI登记号 | NP_075383.1 |
| UniProt登记号 | Q9H9V9 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
