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位置: 首页> 品牌> Sigma-Aldrich> Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

品牌
Sigma-Aldrich
货号
07-947
规格纯度
from rabbit, purified by affinity chromatography
价格
6694.05 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody
histone 4, H4, histone H4, histone cluster 4, H4
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Arginine modifications of histones proteins are mediated by two classes of protein arginine methyltransferases (PRMTs), class I and II. Class I PRMTs place two methyl groups on the one nitrogen atom of the arginine guanidino group, resulting in an asymmetric modification. In contrast class II PRMTs place two methyl groups on two nitrogen atoms of the guanidino group producing a symmetric dimethylation. Although there are exceptions, asymmetric modifications are typically associated with transcription activation while symmetric modifications are most commonly associated with transcriptional repression.

特异性

Broad species cross-reactivity expected based on sequence similarity.
This antibody recognizes Histone H4 symmetrically dimethylated at Arg3.

免疫原

KLH-conjugated linear peptide corresponding to human Histone H4 when symmetrically dimethylated at Arg3.
Epitope: Symmetrically dimethylated Arg3

应用

Use Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody (rabbit polyclonal antibody) validated in WB, ICC, DB to detect dimethyl-Histone H4 (Arg3) Symmetric also known as histone H4, histone cluster 4 H4.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H4 in HeLa and A431 cells.

Peptide Dot Blot Analysis: 0.5 ?g/mL of this antibody detected Histone H4 dimethyl Arg3 (symmetric) peptides, but did not detect Histone H4 dimethyl Arg3 (assymmetric) peptide, other modified peptides, or non-methyated histone H4 peptides.

质量

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 0.1 ?g/mL of this antibody detected Histone H4 on 10 ?g of HeLa acid extract.

目标描述

~13 kDa observed. Crossreacting band at ~58 kDa has been observed in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa acid extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity rat, human, mouse
technique(s) dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI登记号 NP_001029249
UniProt登记号 P62805
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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