半岛bd体育手机客户端 说明
一般描述
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.
特异性
Recognizes VEGFR2/Flk-1/KDR when phosphorylated on Tyrosine 1054 (human).
免疫原
Synthetic peptide encompassing
应用
Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr1054) for use in WB.
Research Category
Signaling
质量
Western Blot Analysis:
This lot detected VEGFR2 phosphorylated on Tyr1054 at a 1:1,000 dilution in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min. Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
目标描述
152 kDa
外形
Double affinity purified
Affinity purified rabbit polyclonal IgG in PBS with 0.05% NaN3 and 50% glycerol.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
CSF-1 treated PAE cell lysate, transfected with CSF/KDR.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human, zebrafish |
species reactivity (predicted by homology) | mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology), chicken (based on 100% sequence homology),Danio(based on 100% sequence homology), rat (based on 100% sequence homology), primate (based on 100% sequence homology) |
manufacturer/tradename | Upstate? |
technique(s) | western blot: suitable |
NCBI登记号 | NM_002253.2 |
UniProt登记号 | P35968 |
运输 | dry ice |
Gene Information | human ... KDR(3791) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich