您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-phospho-Phospholamban (Ser16) Antibody

Anti-phospho-Phospholamban (Ser16) Antibody

品牌
Sigma-Aldrich
货号
07-052
规格纯度
Upstate?, from rabbit
价格
7197.66 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-phospho-Phospholamban (Ser16) Antibody
Cardiac phospholamban
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

特异性

Predicted to cross-react with human, mouse, rat, rabbit, pig and dog.
Recognizes phosphorylated phospho-lamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates.

免疫原

KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.

应用

Research Category
Metabolism

Signaling
This Anti-phospho-Phospholamban (Ser16) Antibody is validated for use in WB for the detection of phospho-Phospholamban (Ser16).
Research Sub Category
Muscle Physiology

General Post-translation Modification

质量

Routinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA.

Western Blot Analysis:
0.5-2 ?g/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 ?g PKA (Catalog # 14-114) for 10 minutes.
DO NOT BOIL CARDIAC PREPARATION.
Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.

目标描述

Varies

外形

Immunoaffinity Chromatography
Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析说明

Control
Bovine cardiac preparation, treated with PKA.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity purified immunoglobulin
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity mouse, human, canine, pig, rat, bovine, rabbit
manufacturer/tradename Upstate?
technique(s) western blot: suitable
同位素/亚型 IgG
NCBI登记号 NP_002658
UniProt登记号 P26678
运输 dry ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map