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位置: 首页> 品牌> Sigma-Aldrich> Anti-monomethyl-Histone H3 (Lys9) Antibody

Anti-monomethyl-Histone H3 (Lys9) Antibody

品牌
Sigma-Aldrich
货号
07-450
规格纯度
Upstate?, from rabbit
价格
6694.05 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-monomethyl-Histone H3 (Lys9) Antibody
H3K9me1, Histone H3 (mono methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

特异性

Recognizes monomethylated histone H3 (Lys9), Mr ~17 kDa.
Broad species cross-reactivity is expected based on sequence homology.

免疫原

Epitope: Lys9
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKST… in which meK corresponds to monomethyl-lysine at residue 9 of human histone H3.

应用

Anti-monomethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 9 (H3K9me1). This purified antibody is specificity verified by dot blot (DB), published in peer reviewed journals, and has been validated in ICC, PIA & WB.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Peptide Competition:
Specificity confirmed by the ability of 10 μM of the immunizing peptide to abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts (Figure B, Lane 2). No signal reductions were detected with preincubation with 10 μM di- or trimethyl-lysine 9 or di- or trimethyl-lysine 27 modified peptides. Some signal reduction detected after preincubation with 10 μM monomethyl-lysine 27 modified peptide (Figure B, Lane 5, page 2).

Immunocytochemistry & Dot Blot: Reported by an independent laboratory1-4.

质量

Routinely evaluated by Western Blot on acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).

目标描述

17 kDa

外形

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa cell extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 polyclonal
species reactivity mouse, chicken, human
manufacturer/tradename Upstate?
technique(s) dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NM_003493
UniProt登记号 Q16695
运输 wet ice
Gene Information human ... HIST1H3F(8968)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1

Sigma-Aldrich

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