Anti-ECT2 Antibody

半岛bd体育手机客户端 说明

一般描述

Epithelial cell-transforming sequence 2 oncogene (ECT2) is a guanine nucleotide exchange factor or GEF that belongs to the Dbl family of Rho GEFs. ECT2 is composed of 2 BRCT domains within the N-terminus, a DBL-homology domain, and a PH domain. Studies have shown ECT2 to localize to both the cell cortex and central spindles, and that it is a crucial part of cleavage furrow formation during cytokenises. Overexpression of cleaved ECT2, containing only the N-terminal domain, has been shown to cause multinucleation. As a GEF it has been shown to bind specifically to Rac proteins, RhoA, and RhoC.

特异性

This antibody recognizes ECT2 at the
C-terminus.

免疫原

KLH-conjugated linear peptide corresponding to human ECT2 at the C-terminus.
Epitope: C-terminus

应用

Anti-ECT2 Antibody is an antibody against ECT2 for use in WB & IC.
Research Category
Signaling
Western Blot (Snap i.d.) Analysis: 5 ?g/mL from a previous lot detected ECT2 in A549 cell lysate.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory on Heya8 cells. (Der Channing, University of North Carolina at Chapel Hill, NC, channing_der@med.unc.edu)
Research Sub Category
G-proteins

质量

Evaluated by Western Blot in A549 cell lysate.
Western Blot Analysis: 1 ?g/mL of this antibody detected ECT2 on 10 ?g of A549 cell lysate.

目标描述

~ 105 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
A549 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	pig, human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), opossum (based on 100% sequence homology), rabbit (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable western blot: suitable
UniProt登记号	Q9H8V3
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable