Anti-Frizzled-7 Antibody

半岛bd体育手机客户端 说明

一般描述

Frizzled 7 is a multi-pass membrane protein belonging to the G-protein coupled receptor Fz/Smo family. It is a receptor for Wnt signaling, predominantly Wnt11, a crucial factor in the regulation of cellular migration and polarization during vertebrate gastrulation. Highest reported expression is in fetal kidney tissue and adult heart tissue. Lower levels have been observed in brain, placenta, adult heart, fetal lung, and squamous cell esophageal carcinomas.

特异性

Recognizes Frizzled-7

免疫原

KLH-conjugated linear peptide corresponding to human Frizzled-7.
Epitope: Unknown

应用

Western Blot Analysis: A previous lot detected Frizzled-7 on 10 ?g of HEK293 cell lysate and rat brain microsomal fraction tissue lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Frizzled-7 in human ductalin situand GI tract tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Frizzled-7 in A431 cells.
Research Category
Signaling
This Anti-Frizzled-7 Antibody is validated for use in WB, IH, IC for the detection of Frizzled-7.
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blot in mouse brain microsomal fraction tissue lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected Frizzled-7 on 10 ?g of mouse brain microsomal fraction tissue lysate.

目标描述

The calculated molecular weight is 64 kDa Observed at ~83 kDa, most likely due to multiple glycosolation sites.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain microsomal fraction tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse, rat
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
NCBI登记号	NP_003498.1
UniProt登记号	O75084
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable