Anti-acetyl-p53 (Lys320) Antibody

半岛bd体育手机客户端 说明

一般描述

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features:
a) Most of them are missense point mutations giving rise to an altered protein function.
b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

特异性

This antibody recognizes p53 acetylated at Lys320.

免疫原

Epitope: Acetyl Lys 320
KLH-conjugated linear peptide corresponding to p53 at Lys320.

应用

Western Blot Analysis: 5 ?g/mL antibody detected p53 on 10 ?g of A549 cells treated with UV & TSA lysate.

Western Blot (SNAP ID) Analysis: 5 ?g/mL antibody detected p53 on 10 ?g of recombinant proteins.
Research Sub Category
Transcription Factors
Use Anti-acetyl-p53 (Lys320) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect acetyl-p53 (Lys320) also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, p53 antigen.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting using A549 cells treated with UV & TSA.
Western Blotting Analysis: 5 ?g/mL of a representative lot of this antibody detected p53 on 10 ?g of A549 cells treated with UV & TSA lysate.

目标描述

~ 53 kDa observed. Western Blot of A549 cells treated with UV & TSA show bands at ~48 kDa and ~14 kDa which are likely breakdown products of p53 reported for UV treated cells. (Sadji-Ouatas, 2002)

联系

Replaces: 06-915

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Recombinant proteins

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)	western blot: suitable
NCBI登记号	NP_000537
UniProt登记号	P04637
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable