半岛bd体育手机客户端 说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
Histone H3 acetylated at position 9.
although this peptide sequence is identical in a wide range of animal and plant species.
免疫原
KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.
Epitope: Surrounding H3K9ac
应用
Chromatin Immunoprecipitation/ChIP-seq Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys9) in 5 ?g of HeLa cell lysate.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance
Flow Cytometry Analysis: 0.25 ?g of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.
Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot blot (DB), published in peer reviewed journals, and validated in WB, ChIP, ChIP-seq, FC.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 ?g of sodium butyrate treated HeLa cell lysate.
目标描述
~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
联系
Replaces: 04-1003
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
Format: Purified
Protein A purified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | mouse, rat, human |
| manufacturer/tradename | Upstate? |
| technique(s) | ChIP: suitable (ChIP-seq) dot blot: suitable flow cytometry: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| NCBI登记号 | NP_003520 |
| UniProt登记号 | Q16695 |
| 运输 | dry ice |
| Gene Information | human ... HIST1H3F(8968) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
