Anti-phospho-CREB (Ser133) Antibody

半岛bd体育手机客户端 说明

一般描述

CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

特异性

Recognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.

免疫原

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
Epitope: phospho-Ser133

应用

Immunoprecipitation:
10 μL of a previous lot immunoprecipitated phosphorylated CREB from 500 μg of cells treated with 50 μM forskolin.

Immunohistochemistry:
A 1:1000 dilution of previous lots detected phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.

Gel Shift (EMSA):
This antibody has been shown to gel shift by independent labs.
Research Sub Category
Nuclear Receptors
Use Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) is highly published and validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
Research Category
Signaling

质量

Evaluated by western blot on lysates from mouse NIH/3T3 cells treated with 50 mM forskolin.

Western Blot Analysis: This antibody detected CREB when phosphorylated on Ser133 at 1:1,000 dilution in forskolin treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

目标描述

Detects CREB only when phosphorylated on Ser133 (~43 kDa). May recognize ATF-1 (~38 kDa) and CREM (~30 kDa) as the two proteins share significant homology to the phosphorylated immunizing peptide.

外形

Format: Purified
Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.
Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4) 15 mM NaCl, and 0.05% sodium azide with 30% glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Forskolin-stimulated WI-38 nuclear extracts.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	mouse, rat, hamster, human
manufacturer/tradename	Upstate?
technique(s)	electrophoretic mobility shift assay: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG
NCBI登记号	np_604391
UniProt登记号	P16220
运输	wet ice
Gene Information	human ... CREB1(1385)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable