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位置: 首页> 品牌> Sigma-Aldrich> Anti-ITK/EMT/TSK Antibody

Anti-ITK/EMT/TSK Antibody

品牌
Sigma-Aldrich
货号
06-1064
规格纯度
from rabbit, purified by affinity chromatography
价格
4848.93 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-ITK/EMT/TSK Antibody
IL2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Tyrosine-protein kinase ITK/TSK (UniProt: Q08881; also known as EC:2.7.10.2, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk) is encoded by the ITK (also known as EMT, LYK) gene (Gene ID: 3702) in human. ITK/TSK is a protein tyrosine kinase that plays an essential role in regulation of the adaptive immune response. It regulates the development, function, and differentiation of conventional T-cells and non-conventional natural killer T-cells. It contains four zinc binding sites. It s N-terminal PH domain allows it to be recruited to the plasma membrane by an activated PI3 kinase. This domain also contains a proline-rich region (PRR). The adjoining SH3 domain binds to PRR. The SH2 domain is required for binding tyrosine-phosphorylated substrates and the kinase domain located in the C-terminal region is required for tyrosine phosphorylation. ITK/TSK is phosphorylated at Tyr512 in the activation loop of the kinase domain by LCK and subsequent autophosphorylation at Tyr180, which lies within the substrate-binding sequence in the SH3 domain, allows the kinase activation. Mutations in ITK gene are reported to cause lymphoproliferative syndrome 1, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus that can lead to fatal outcome.

特异性

Predicted to cross-react with mouse based on 100% sequence homology.
Recognizes Itk/Emt/Tsk, Mr ~72 kDa.

免疫原

GST-tagged recombinant fragment cvorresponding to the first 270 amino acids from the N-terminal half of human Tyrosine-protein kinase ITK/TSK spanning the PH, SH3 and a small portion of the SH2 domains.

应用

Research Category
Signaling

Inflammation & Immunology
Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated ITK/EMT/TSK in 500 ?g of Jurkat cell lysate.
Research Sub Category
Immunological Signaling
This rabbit polyclonal Anti-ITK/EMT/TSK, Cat. No. 06-1064 is a rabbit polyclonal antibody that targets protein tyrosine kinase ITK/TSK and is tested for use in Immunoprecipitation and Western blotting.

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ITK/EMT/TSK in 10 ?g of Jurkat cell lysate.

目标描述

~71 kDa observed; 71.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: 06-546

外形

Purified rabbit polycloncal in buffer containing PBS with 0.05% sodium azide and 50% glycerol.
Affinity purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial and prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

分析说明

Control
Jurkat cell lysates

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
technique(s) immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号 NP_005537
UniProt登记号 Q08881
运输 wet ice
Gene Information human ... ITK(3702)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2

Sigma-Aldrich

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