Anti-Tet2 Antibody

半岛bd体育手机客户端 说明

一般描述

Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).

免疫原

KLH-conjugated linear peptide corresponding to human Tet2.

应用

Anti-Tet2 detects levels of Tet2 proteins & has been published & validated for use in WB, IHC & ICC.
Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected Tet2 in rat medulla tissue.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tet2 in human H9 Embryonic Stem cells.

质量

Evaluated by Western Blotting in mouse Embryonic Stem cells.

Western Blotting Analysis: 1 ?g/mL of this antibody detected Tet2 in 10 ?g of mouse Embryonic Stem cells (Note: Isoforms 2 and 3 may observed at low concentrations or longer exposure times, while isoform 1 may be detected at higher concentrations or longer exposure times in certain cell lysates.).

目标描述

~140 kDa observed. Three isoforms at 223 kDa (isoform 1), 130 kDa (isoform 2), and 133 kDa (isoform 3) are known to exist. This antibody should recognize all three isoforms based on sequence homology. An uncharacterized band may be observed at ~36 kDa in some cell lysates. (Note: Isoforms 2 and 3 may observed at low concentrations, while isoform 1 may be detected at higher concentrations in certain cell lysates.)

外形

Affinity purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, rat, mouse
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_060098
UniProt登记号	Q6N021
运输	wet ice
Gene Information	human ... TET2(54790)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable