Anti-CRKL Antibody

半岛bd体育手机客户端 说明

一般描述

Crk-like protein (CRKL) is a 39 kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.

免疫原

KLH-conjugated linear peptide corresponding to human CRKL.

应用

Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 0.25 ?g/mL from a representative lot detected CRKL in 10 ?g of NIH3T3 and L6 cell lysate.
Immunoprecipitation Analysis: 10 ?g from a representative lot immunoprecipitated CRKL in 500 ?g of Jurkat cell lysate.
Detect Crk-like protein using this rabbit polyclonal antibody, Anti-CRKL Antibody validated for use in western blotting & IP.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.25 ?g/mL of this antibody detected CRKL in 10 ?g of Jurkat cell lysate.

目标描述

~39 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, rat, mouse
species reactivity (predicted by homology)	rhesus macaque (based on 100% sequence homology)
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_005198
UniProt登记号	P46109
运输	wet ice
Gene Information	human ... CRKL(1399)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable