半岛bd体育手机客户端 说明
一般描述
GAD65 is a group II decarboxylase enzyme that catalyzes the biosynthesis of GABA from glutamate with the aid of the cofactor, pyridoxal 5′-phosphate. GAD65 localizes at presynaptic GABAergic terminals and is reported to supply local vesicular pools of GABA facilitating transient inhibitiory neurotransmission, although GAD65 may also play a role in more sustained tonic inhibitory signals. It is present as a dimer of alpha and beta subunits and is regulated by alternative splicing, which may produce the GAD67 isoform. GAD65 also undergoes post-translational modifications such as palmitoylation of N-terminal cysteine residues and phosphorylation of multiple alpha-subunit serine residues that enable membrane-anchoring and other vesicular activities. GAD65 has been implicated in neurological conditions resulting from failure of inhibitory neurotransmission such as Huntington′s disease and epilepsy. In addition to its CNS presence, GAD65 also produces GABA in pancreatic beta cells and dysfunction in this area may contribute to the pathogenesis of insulin-dependent diabetes mellitus.
免疫原
KLH-conjugated linear peptide corresponding to human GAD65.
应用
Western Blot Analysis: 1 ?g/mL from a representative lot detected GAD65 on 10 ?g of rat brain membrane tissue lysate. Western Blot performed without Tween.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-GAD Antibody is an antibody against GAD65 for use in Western Blotting. See below for more information about this anti GAD antibody.
质量
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 1 ?g/mL of this antibody detected GAD65 on 10 ?g of rat brain membrane tissue lysate.
目标描述
~59/63 kDa observed. The calculated molecular weight is 65 kDa, however GAD65 has been shown as a ~59/63 doublet in western blots. (Christgau, S., et al. (1991). Journal of Biological Chemistry. 266(31):21257-21264.) An uncharacterized band appears at ~38 kDa in some lysates.
外形
Purified Rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Rat brain membrane tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | rat, mouse, human, pig |
| species reactivity (predicted by homology) | porcine (based on 100% sequence homology), canine (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI登记号 | NP_000809 |
| UniProt登记号 | Q05329 |
| 运输 | wet ice |
| Gene Information | human ... GAD2(2572) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
