Anti-Spastin Antibody

半岛bd体育手机客户端 说明

一般描述

Spastin is a distinct microtubule-severing protein. Autosomal dominant mutations in the SPG4 locus corresponding to spastin are the most common cause of hereditary spastic paraplegia (HSP), a neurodegenerative disease that afflicts the adult corticospinal tracts.

免疫原

Recombinant protein corresponding to human Spastin with 4 isoforms.

应用

Western Blotting Analysis: A representative lot from an independent laboratory detected Spastin in myc-Spastin transfected HeLa and MRC5 cell lysates (Connell, et al. (2009). Traffic. 10:42-56).
Detect Spastin using this rabbit polyclonal antibody, Anti-Spastin Antibody validated for use in western blotting & ICC.

质量

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Spastin in 10 ?g of L6 cell lysate.

目标描述

~ 68, 64, 58, 55 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing at ~68 kDa, ~64 kDa, ~58 kDa, and ~55 kDa Spastin has shown in some western blots that four isoforms [full length (68 kD), exon 4-deleted full length (64 kD), short form (58-60 kD) and exon 4-deleted short form (55 kD)] are expressed in mammalian cells. Ths pAb detects bands corresponding to the size of these four isoforms in HeLa, MRC5 human lung fibroblast and NSC34 mouse lower motor neuron neuroblastoma fusion cell lines (Connell, et al. (2009). Traffic. 10:42-56).

外形

Unpurified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human, rat, mouse
technique(s)	immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_055761
UniProt登记号	Q9UBP0
运输	wet ice
Gene Information	human ... SPAST(6683)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1