Anti-Smad4/DPC4 Antibody

半岛bd体育手机客户端 说明

一般描述

SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-β, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors.

特异性

This antibody recognizes Smad4 at the SAD region.

免疫原

GST-tagged recombinant protein corresponding to human Smad4 at the SAD region.
Epitope: SAD region

应用

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Smad4 in NIH/3T3, HeLa, and A431 cells.
Research Sub Category
Transcription Factors
Use Anti-Smad4/DPC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Smad4/DPC4 also known as Mothers against decapentaplegic homolog 4, SMAD 4, Smad4, hSMAD4.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 0.1 ?g/mL of this antibody detected Smad4 on 10 ?g of HepG2 cell lysate.

目标描述

~68 kDa observed.

联系

Replaces: 04-1033

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HepG2 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, human, rat
species reactivity (predicted by homology)	chimpanzee (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_005350.1
UniProt登记号	Q13485
运输	wet ice
Gene Information	human ... SMAD4(4089)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable