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Anti-ICAT Antibody

品牌
Sigma-Aldrich
货号
ABC939
规格纯度
from rabbit, purified by affinity chromatography
价格
5688.25 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-ICAT Antibody
Beta-catenin-interacting protein 1, ICAT, Inhibitor of beta-catenin and Tcf-4
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Beta-catenin-interacting protein 1 (UniProt Q9NSA3; also known as Beta-catenin-interacting protein ICAT, Inhibitor of beta-catenin and Tcf-4) is encoded by the CTNNBIP1 (also known as ICAT) gene (Gene ID 56998) in human. Inhibitor of beta-catenin and TCF (ICAT) negatively regulates T-cell factor/lymphoid enhancer factor (TCF/LEF)–beta-catenin transcriptional activity by competing against TCF/LEF for beta-catenin binding. ICAT gene knockout leads to premature death of newborn mice with gut, kidney, and craniofacial anomalies, implicating an important role of ICAT in epithelial establishment/maintenance and neural crest cell differentiation. The motility of melanoma cells through 3D matrices in vitro relies on two interconvertible modes of movements. ICAT is reported to suppress the expression of developmentally downregulated 9 (NEDD9) of Wnt/beta-catenin signaling pathway in human Lu1205 melanoma cells, resulting in a shift from the Rac-dependent mesenchymal movement to the Rho/ROCK-dependent amoeboid motility in vitro and enhanced colonization of transplanted Lu1205 in the lungs of nude mice in vivo.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human ICAT.
Epitope: C-terminus

应用

Research Sub Category
Apoptosis - Additional
Immunoprecipitation Analysis: A representative lot co-immunoprecitated beta-catenin with ICAT from lysates of five human melanoma cell lines (Lu1205, WM852, Dauv-1, Mel501, and Skmel3) (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
Western Blotting Analysis: A representative lot detected ICAT in Lu1205, Mel501, human melanoma cells, and Skmel3 (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
Detect ICAT/CTNNBIP1 using this rabbit Polyclonal Anti-ICAT Antibody, Cat. No. ABC939, validated for use in Immunocytochemistry, Western Blotting and Immunoprecipitation.
Research Category
Apoptosis & Cancer

质量

Evaluated by Immunocytochemistry in A431, HeLa, HUVEC and cells.

Immunocytochemistry Analysis: 4 ?g/mL of this antibody detected ICAT in A431, HeLa, HUVEC cells.

目标描述

~9 kDa calculated

外形

Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 purified antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
species reactivity (predicted by homology) zebrafish (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology),Xenopus(based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s) immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号 NP_064633
UniProt登记号 Q9NSA3
运输 wet ice
Gene Information human ... CTNNBIP1(56998)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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