Anti-Grainyhead-Like 3/GRHL3 Antibody

半岛bd体育手机客户端 说明

一般描述

Grainyhead-like protein 3 homolog (UniProt Q8TE85; also known as Sister of mammalian grainyhead, Transcription factor CP2-like 4) is encoded by the GRHL3 (also known as SOM, TFCP2L4, VWS2) gene (Gene ID 57822) in human. Mammalian grainyhead-like (GRHL1, GRHL2, and GRHL3) genes code for transcription factors and are named after their drosophila homolog grainyhead (GRH). These factors show tissue- and developmental stage-dependent expression patterns. Grainyhead-like 3 (GRHL3) is reported to play a protective role against squamous cell carcinoma (SCC) of the skin. Tumor suppressor phosphatase and tensin homolog (PTEN) is a direct target of GRHL3, Grhl3-knockdown in mice causes skin tumor formation as a result of reduced Pten expression. Consistently, downregulated GRHL3 and PTEN levels are found in human SCC skin samples. In addition, GRHL3 is a positive regulator of E-cadherin expression during mesenchymal epithelial transition (MET) in non-tumorigenic mammary gland cells, as well as in breast and lung cancer cells. High GRHL3 expression is found in early stages of breast cancer and the expression is greatly reduced in advanced stages of breast cancer.

特异性

Expected to react with all five spliced isoforms of human grainyhead-like protein 3 reported by UniProt (Q8TE85).

免疫原

Epitope: Internal (C-terminal half).
KLH-conjugated linear peptide corresponding to an internal sequence from the C-terminal half of human Grainyhead-Like 3/GRHL3.

应用

Research Sub Category
Transcription Factors
Western Blotting Analysis: 1 ?g/mL of this antibody from a representative lot detected both endogenous Grainyhead-Like 3/GRHL3 and a myc-tagged GRHL3 in 50 ?g of lysate from transfected HEK293 cells.
Immunoprecipitation Analysis: 5.0 ?g of this antibody from a representative lot immunorecipitated both the endogenous and an epitopically tagged human GRHL3 transiently expressed in HEK293 cells (Courtesy of Professor Judith Haendeler, IUF-Leibniz Research Institute, University of Duesseldorf, Germany).
Immunocytochemistry Analysis: 4.0 ?g/mL of this antibody from a representative lot detected GRHL3 in paraformaldehyde-fixed, Triton X-100-permeablized HUVECs (Courtesy of Professor Judith Haendeler, IUF-Leibniz Research Institute, University of Duesseldorf, Germany).
Western Blotting Analysis: 1.0 ?g/mL from a representative lot detected both the endogenous and an epitopically tagged human GRHL3 transiently expressed in HEK293 cells (Courtesy of Professor Judith Haendeler, IUF-Leibniz Research Institute, University of Duesseldorf, Germany).
Research Category
Stem Cell Research
Anti-Grainyhead-Like 3 Antibody is an antibody against GRHL3 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 ?g/mL of this antibody detected Grainyhead-Like 3/GRHL3 in 10 ?g of Jurkat cell lysate.

目标描述

~62 kDa observed. 70.35 kDa (isoform 1), 68.35 kDa (isoform 2), 62.38 kDa (isoform 3), 57.05 kDa (isoform 4), and 67.70 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.75

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_937817
UniProt登记号	Q8TE85
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable