Anti-FAM60A Antibody

半岛bd体育手机客户端 说明

一般描述

FAM60A, also known as Protein FAM60A, or Tera protein homolog, and encoded by the gene FAM60A/C12orf14, is a subunit of the Sin3 deacetylase complex (Sin3/HDAC) that is important for the repression of genes. The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. FAM60A specifically impacts genes encoding components of the TGF-beta signaling pathway and promoters of important proteins like cyclin D1. FAM60A activity peaks during G1 and S phases of the cell cycle and is up regulated in many carcinoma and tumor tissues. Additionally, loss of FAM60A leads to a change in cell morphology, an increase in cell migration, increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry.

免疫原

KLH-conjugated linear peptide corresponding to human FAM60A.

应用

Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 2.0 ?g/mL from a representative lot detected FAM60A in 10 ?g of A549 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FAM60A in human prostate cancer and human breast cancer.
Research Category
Apoptosis & Cancer
Anti-FAM60A Antibody detects level of FAM60A & has been published & validated for use in FAM60A.

质量

Evaluated by Western Blotting in transfected FAM60A cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected FAM60A in 10 ?g of transfected FAM60A cell lysate.

目标描述

~35 kDa observed. Although endogenous expected and detected ~ 25kDa , transfected seen at ~35kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	rat (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable western blot: suitable
NCBI登记号	NP_001129283
UniProt登记号	Q9NP50
运输	wet ice
Gene Information	human ... FAM60A(58516)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable