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Anti-Brd4 Antibody

品牌
Sigma-Aldrich
货号
ABE1391
规格纯度
serum, from rabbit
价格
5688.25 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Brd4 Antibody
Bromodomain-containing protein 4, Mitotic chromosome-associated protein, MCAP, Brd4
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Bromodomain-containing protein 4 (Brd4), or Mitotic chromosome-associated protein (MCAP), is a chromatin reader protein and kinase that recognizes and binds acetylated histones and plays a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Brd4 remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, Brd4 plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Brd4 is widely expressed and localized to the nucleus and acetylated chromatin. Brd4 also plays a large role in many cancers, and through its regulation of transcription, Brd4 is important in multiple cellular processes including DNA damage responses, aging, and development.

特异性

Recognizes human Brd4

免疫原

Epitope: Region between amino acids 150 to 290 of human Brd4
Recombinant protein corresponding to Mouse Brd4 short form.

应用

Research Sub Category
Chromatin Biology

Histones
This Anti-Brd4 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunocytochemistry for the detection of Brd4.
Western Blotting Analysis: A representative lot of this antibody was reported to detect Brd4 protein in a HeLa cell lysate (Alsarraj J., et al. (2013). Also see Anup, D., et al. (2003) and Mochizuki, K., et al. (2008) for additional data using mouse embyronic fibroblasts and NIH3T3 cells.

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP using chromatin from NIH3T3 cells. See Mochizuki, K., et al. (2008) and Anup, D., et al. (2009).

Immunocytochemistry: A representative lot of this antibody was reported to work in ICC. See Anup, D., et al. (2000) and Anup, D., et al. (2009).

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HEK293 cells.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected Brd4 in 10 ?g of HEK293 cell lysate.

目标描述

~ 100 kDa observed
This antibody primarily detects 100 kd short form of Brd4 and to lesser extent larger 180 kD form (see Alsarraj J., et al. (2013 for additional details on performance of this antibody).

外形

Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 serum
antibody product type primary antibodies
克隆 polyclonal
species reactivity human
species reactivity (predicted by homology) mouse
technique(s) ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI登记号 NP_065254
UniProt登记号 Q9ESU6
运输 dry ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1

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