Monoclonal Anti-Calreticulin antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice. Calreticulin is a molecular chaperones in endoplasmic reticulum lumen. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins, and misfolded proteins. Calreticulin is a protein binding Ca²⁺ions. It is encoded by the CALR gene in humans.
Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice.

应用

Monoclonal Anti-Calreticulin antibody produced in mouse is suitable for the following applications:

• Flow cytometry
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Western blotting

生化/生理作用

It is believed to play a critical role in quality control processes during protein synthesis and folding. Increased expression of calreticulin increases Ca²⁺storage capacity of the ER. It also appears to modulate store-operated Ca²⁺-influx, and to alter Ca²⁺transport by the sarcoplasmic/ER Ca²⁺-ATPase (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. In contrast, calreticulin-deficient cells show increased resistance to apoptosis. Calreticulin gene disruption leads toimpairment of cardiac development. Loss of calreticulin function favors ubiquitin-proteosome activity. It plays a critical role in Ca²⁺homeostasis.
Calreticulin and Its variants causes differential effects on thrombopoiesis. Somatic mutations of calreticulin (CALR) gene causes thrombocythemia (ET) and primary myelofibrosis (PMF).

外形

Culture supernatant solution containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.44

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	tissue culture supernatant
antibody product type	primary antibodies
克隆	TO-11, monoclonal
形式	buffered aqueous solution
分子量	antigen ~50 kDa
species reactivity	human
technique(s)	flow cytometry : suitable immunocytochemistry: suitable immunohistochemistry : suitable indirect ELISA : suitable western blot : 1:50-1:100 using total cell extract of HeLa cells
同位素/亚型	IgG1
UniProt登记号	P27797
运输	dry ice
储存温度	?20℃
Gene Information	human ... CALR(811)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)