Anti-phospho-ASK1 (pSer⁹⁶⁶) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as apoptosis signal-regulating kinase 1 (ASK1), is encoded by the gene mapped to human chromosome 6q22.33. The encoded protein belongs to the mitogen-activated protein kinase family. Porcine MAP3K5, which shares 97 % sequence identity with human MAP3K5, is characterized with a domain of unknown function (DUF4071) and a protein kinase domain.
Apoptosis signal-regulating kinase 1 (ASK1) is a serine/threonine protein kinase. It belongs to the MAPKKK (mitogen-activated protein kinase) family. This gene is located on human chromosome 6q23.

免疫原

The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.

Immunogen Range:932-981

应用

Anti-phospho-ASK1 (pSer966) antibody has been used in western blotting.
Anti-phospho-ASK1 (pSer966) antibody produced in rabbit has been used in immunoblot analysis.

生化/生理作用

Apoptosis signal-regulating kinase 1 (ASK1) is linked with apoptosis in cancer and heart tissues. It act as an upstream regulator of p38 and JNK (c-Jun N-terminal kinase) in cardiomyocytes death. Mutations in MAP3K5 gene result in β-thalassemia disease severity and hydroxyurea treatment efficacy.
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), is one of the vital genes involved in the mitogen-activated protein kinase (MAPK) signaling cascade, which plays a crucial role in the cytoplasmic transduction of extracellular signals. Apoptosis signal-regulating kinase 1 (ASK1)-dependent mitochondrial apoptosis pathway, activated by 4SC-202, which is a novel class I HDAC inhibitor (HDACi), potentially stops hepatocellular carcinoma (HCC) cell proliferation. The encoded protein in response to oxidative stress, endoplasmic reticulum stress, infection and calcium influx plays a vital role in intracellular signaling pathways and biological functions including cell death, differentiation and inflammation. Genomic variation in MAP3K5 indicates β-thalassemia disease severity and hydroxyurea (HU) treatment efficiency. ASK1 plays a vital role in inhibition of the adipocytic differentiation process of the tumor cells and functions as an efficient therapeutic target for several human diseases including malignant fibrous histiocytomas.

特点和优势

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外形

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 154 kDa
species reactivity	rat, human, mouse
浓度	~1 mg/mL
technique(s)	ELISA: 1:20000 immunohistochemistry: 1:50-1:100 western blot: 1:500-1:1000
NCBI登记号	Q99683
UniProt登记号	Q99683
运输	wet ice
储存温度	?20℃
Gene Information	human ... MAP3K5(4217)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable