Anti-phospho-Akt (pThr³⁰⁸) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

The AKT/protein kinase B gene has three homologues, namely PKBα (Akt1), PKBβ (Akt2), and PKBγ (Akt3), which are localized to human chromosome 14q32, 19q13, and 1q44, respectively. The members of AKT family contain an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker, and a carboxyl-terminal kinase domain. The AKT1 and AKT2 genes are expressed highly in insulin-responsive tissues, such as brown fat.

免疫原

The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Thr308.

Immunogen Range:276-325

应用

Anti-phospho-Akt (pThr308) antibody produced in rabbit has been used in immunoblotting.

生化/生理作用

AKT is activated in a multistep process that involves its translocation across the membrane and its phosphorylation. The 3′-phosphorylated phosphoinositides, 3,4,5-trisphosphate (PI-3,4,5-P3) and PI-3,4,-P2 produced by PI3K bind to the PH (pleckstrin homology) domain and this binding facilitates the localization of AKT kinases to the plasma membrane. Once localized, PDK1 (3-phosphoinositide-dependent kinase) phosphorylates Thr-308/309 residue on the AKT molecule, which is essential for its activation. Another residue, Ser- 473/474 on AKT, is also phosphorylated by PDK2. This phosphorylation, although not necessary, increases the activity of AKT. It plays a role in cell survival by regulating the effect of growth factors. AKT may facilitate phosphorylation and inactivation of glycogen synthase kinase-3 by insulin. In adipocytes, it may be involved in the activation of glucose transporter translocation. It is involved in the regulation of several proteins that are involved in cellular processes, such as metabolism, apoptosis, and proliferation. Variations in PI3K-AKT signaling have been observed in several types of cancer, such as human gastric cancer, prostate and breast cancers.

特点和优势

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外形

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 55 kDa
species reactivity	mouse, rat, human
浓度	~1 mg/mL
technique(s)	ELISA: 1:10000 immunohistochemistry: 1:50-1:100 western blot: 1:500-1:1000
NCBI登记号	P31749 P31751
UniProt登记号	P31751
运输	wet ice
储存温度	?20℃
Gene Information	human ... AKT1(207), AKT2(208)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable