Anti-CUX1 Antibody, a.a. 861

半岛bd体育手机客户端 说明

一般描述

Homeobox protein cut-like 1 (CUX1) is also known as CCAAT displacement protein (CDP) and Homeobox protein cux-1. CUX1 is thought to repress expression of developmentally regulated genes involved in mammalian development, likely through the prevention of CCAAT factor binding at promoters. CUX1 also represses T-cell receptor (TCR) beta enhancer function by binding to matrix attachment region beta (MARbeta), and is a component of the nf-munr repressor, which binds to matrix attachment regions (MARs) of the immunoglobulin heavy chain enhancer.

特异性

This antibody recognizes the region of amino acids beginning at 861 of CUX1. This antibody is expected to cross react with all known isoforms of CUX1.
Other homologies: Mouse (95% sequence homology).

免疫原

Epitope: Region of amino acids beginning at 861
GST-tagged recombinant protein corresponding to a region of amino acids beginning at 861 of human CUX1.

应用

Research Sub Category
Transcription Factors
Immunoprecipitation Analysis: 1.25 ?g from a representative lot immunoprecipitated CUX1 in 500 ?g of HeLa nuclear extract.

Alexa Fluor^?is a registered trademark of Life Technologies .
Detect Cux1 using this rabbit polyclonal antibody, Anti-CUX1 Antibody, a.a. 861 validated for use in western blotting & IP.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.25 ?g/mL from a representative lot detected CUX1 in 10 ?g of HeLa nuclear extract.

目标描述

~200 kDa and ~110 kDa observed. Uniprot describes 11 different isoforms. A full length protein has been described at 200 kDa and is proteolytically cleaved into the p150, p110, p90, and p80 isoforms. Additionally, the p75 isoform is the result of alternative splicing (Sansregret, L, et al. (2008). Gene. 412(1-2):84-94.). Uncharacterized band(s) may be observed in some cell lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa nuclear extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_853530
UniProt登记号	P39880
运输	wet ice
Gene Information	human ... CUX1(1523)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable