半岛bd体育手机客户端 说明

一般描述

Calsequestrin is a major Ca²⁺binding protein in cardiac and skeletal muscle.
Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.

免疫原

synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1.

应用

Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.
Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)

生化/生理作用

Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca²⁺binding glycoprotein, which functions as an internal Ca²⁺store in the lumen of the sarcoplasmic reticulum (SR).

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.44

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~55 kDa
species reactivity	human, rat
species reactivity (predicted by homology)	mouse
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
浓度	~1.0 mg/mL
technique(s)	western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2 western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)
UniProt登记号	O14958
运输	dry ice
储存温度	?20℃
Gene Information	human ... CASQ2(845) mouse ... Casq2(12373) rat ... Casq2(29209)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)