Anti-BDNF antibody, Mouse Monoclonal

半岛bd体育手机客户端 说明

一般描述

Anti-BDNF antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the BDNF129-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Brain-derived neurotrophic factor (BDNF), also known as Abrineurin, is a secreted dimeric protein, which belongs to the neurotrophin family of growth factors, along with nerve growth factor (NGF) and neurotrophins 3, 4/5 and 6. BDNF is widely expressed in the central nerve system (CNS).

免疫原

Synthetic peptide corresponding to a region located in the mature human BDNF protein conjugated to KLH

应用

Anti-BDNF antibody, Mouse Monoclonal has been used in:

• immunoblotting
• immunohistochemistry
• immunoprecipitation
• immunocytochemistry

生化/生理作用

Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis through the specific receptor tropomyosin-related kinase B (TrkB). Changes in BDNF expression have been extensively investigated in depression, drug addition, schizophrenia, bipolar and anxiety disorders, Huntington′s disease and Rett syndrome. In addition, BDNF is associated with Alzheimer′s disease (AD) and its reduced levels were detected in post-mortem brain tissues of AD patients. It can exert profound effects on development, morphology, synaptic plasticity and brain functions.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	BDNF129-13, monoclonal
形式	buffered aqueous solution
分子量	~12 kDa
species reactivity	human
浓度	~1.0 mg/mL
technique(s)	immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein immunocytochemistry: suitable immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein
同位素/亚型	IgG2b
UniProt登记号	P23560
运输	dry ice
储存温度	?20℃
Gene Information	human ... BDNF(627)