Anti-Atg4D Antibody

半岛bd体育手机客户端 说明

一般描述

Atg4D (autophagy-related protein 4D) is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis.

特异性

This antibody recognizes Atg4D at the N-terminus.

免疫原

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Atg4D.

应用

Anti-Atg4D Antibody is an antibody against Atg4D for use in WB, IH(P).
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution of a previous lot detected Atg4D in human testicular cancer tissue.

Western Blot Analysis: 1 ?g/mL from a representative lot detected Atg4D on 10 ?g of human fetal skeletal muscle tissue, NIH/3T3, and L6 cell lysates.

质量

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.05 ?g/mL of this antibody detected Atg4D in 10 ?g of A431 cell lysate.

目标描述

~56 kDa observed.
The calculated molecular weight is 53 kDa; however, Atg4D has been shown as a ~55 kDa band in western blots (Li, M., et al. (2011). J Biol Chem. 286(9):7327-7338.)

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
A431 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, rat, human
species reactivity (predicted by homology)	rhesus macaque (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_116274
UniProt登记号	Q86TL0
运输	wet ice
Gene Information	human ... ATG4D(84971)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable