Anti-Aprataxin Antibody

半岛bd体育手机客户端 说明

一般描述

Aprataxin (UniProt Q7Z2E3; also known as FHA-HIT, Forkhead-associated domain histidine triad-like protein) is encoded by the APTX (also known as AOA, AOA1, AXA1, EAOH, EOAHA) gene (Gene ID 54840) in human. Aprataxin contains an N-terminal forkhead-associated (FHA; a.a. 38-87) domain akin to that found in the DNA end damage repair factor polynucleotide kinase phosphatase (PNKP), followed by a histidine triad (HIT; a.a. 182-287) domain found in nucleoside hydrolases and transferases, and a C-terminal C2H2 type Zinc finger domain. Aprataxin exhibits both DNA- and RNA-binding activity and plays a role in reversing toxic 5′-adenylated (5′-AMP) DNA lesions generated by DNA ligases when encountering nicked DNA structures with abnormal DNA termini. Aprataxin functions as a DNA ligase proofreader to directly reverse damaged 5′-adenylated termini of DNA strand breaks that have been subjected to DNA damage-induced abortive processing by DNA ligases. Ataxia with oculomotor apraxia type 1 (AOA1) is an autosomal recessive neurological syndrome caused by an impairment in DNA repair as a result of APTX gene mutations. The related type 2 disorder AOA2 is caused by a Senataxin-deficiency due to SETX gene mutations.

特异性

Expected to react with all 13 human aprataxin spliced isoforms reported by UniProt (Q7Z2E3-1 through Q7Z2E3-13).

免疫原

Recombinant full-length human aprataxin.

应用

Anti-Aprataxin Antibody is an antibody against Aprataxin for use in Western Blotting, Immunoprecipitation.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western Blotting Analysis: A representative lot detected wild-type and the V263G mutant form of recombinant human aprataxin (Kijas, A.W., et al. (2006). J. Biol. Chem. 281(20):13939-13948).
Western Blotting Analysis: A representative lot detected aprataxin in lysates from HeLa and control lymphoblastoid cell lines (C3ABR and C2ABR), but not from AOA1 cell lines (L939, L938) derived from patients with the neurological disorder ataxia with oculomotor apraxia type 1 (AOA1) (Becherel, O.J., et al. (2006). Hum. Mol. Genet. 15(14):2239-2249).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated nucleolar proteins nucleolin and nucleophosmin with aprataxin from C3ABR lymphoblastoid cell lysate (Becherel, O.J., et al. (2006). Hum. Mol. Genet. 15(14):2239-2249).
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aprataxin in 10 ?g of HeLa cell lysate.

目标描述

~43 kDa observed.

外形

Unpurified
Rabbit polyclonal antibody in serum with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human
species reactivity (predicted by homology)	mouse (based on high sequence homology), rat (based on high sequence homology), nonhuman primates (based on high sequence homology), porcine (based on high sequence homology), bovine (based on high sequence homology)
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_001182177
UniProt登记号	Q7Z2E3
运输	dry ice
Gene Information	human ... APTX(54840)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable