Anti-Cullin-4A antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Cullin-4 (Cul4) proteins are the core components of ubiquitin E3 ligases. Mammalian cells express two distinct but highly homologous cullin-4 proteins, cullin-4A and cullin-4B.
Cullin-4A is a protein encoded by CUL4A gene in humans.

应用

Anti-Cullin-4A antibody produced in rabbit is used for Immunofluorescence at a concentration of 10-20mg/mL using HeLa cells and is also used for western blotting at a concentration of 1-2μg/mL using HEK-293T cells expressing human Cullin-4A.

生化/生理作用

Cullin-4 (Cul4) proteins are components of ubiquitin E3 ligases that regulate cell cycle, genome stability, DNA replication and transcription. CUL4-based E3 ligase plays an important role for the repression of photomorphogenesis in plants (likeArabidopsis thaliana).
Cullin-4A (Cul4A) is involved in identifying specific targets for ubiquitylation. The Cul4A gene is known to be amplified and up-regulated in breast carcinoma and many other tumor types. Cul4A associates with damage-specific DNA binding proteins (DDB1 and DDB2). It functions as substrate recruiting factors for Cul4A-Roc1 ligases. Cul4A is known to ubiquitylate several target proteins including DDB1/DDB2, CDT1 (chromatin licensing and dna replication factor 1), DNA repair protein complementing XP-C cells (XPC), histone H2A, H3 and H4. In response to DNA damage Cul4A complex regulates protein stability of p53 and mouse double minute 2 homolog (MDM2)/ human double minute 2 homolog (HDM2).

外形

solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~89 kDa
species reactivity	human
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
浓度	~1.5 mg/mL
technique(s)	indirect immunofluorescence: 10-20 μg/mL using HeLa cells western blot: 1-2 μg/mL using HEK-293T cells expressing human Cullin-4A
UniProt登记号	Q13619
运输	dry ice
储存温度	?20℃
Gene Information	human ... CUL4A(8451)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)