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Anti-Cofilin antibody produced in rabbit

品牌
Sigma-Aldrich
货号
C8736
规格纯度
IgG fraction of antiserum, buffered aqueous solution
价格
4330.86 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-Cofilin antibody produced in rabbit
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Cofilin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filamentsin vitro. In mammals it has two isoforms: non-muscle (NM-CF, CF-L1) and muscle (M-CF, CF-L2). The protein is ubiquitiously present in tissues of eukaryotes and is especially abundant in neuronal tissues. It can shuttle between the cytoplasm and the nucleus in response to various stresses or signals, and may translocate from the cytoplasm to the plasma membrane in various cells.
Cofilin is a member of the actin depolymerizing factor/cofilin family. Among all members, cofilin-1 is the predominant isoform. It is located on human chromosome 11q13.

特异性

Reacts with muscle and non-muscle cofilin.

免疫原

Synthetic peptide corresponding to human cofilin sequence, with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

应用

Anti-Cofilin antibody has been used:

  • for immunostaining of chick neurons. It is used as a primary antibody
  • for western blotting of protein isolated from mouse hippocampi cells, rat brain samples, human acute lymphoblastic T-cell line, human brain samples, head and neck squamous cell carcinoma cell line, HEK293T cells and renal epithelial cell lines
  • for immunofluorescence studies in tissue samples from human brain

生化/生理作用

Cofilin binds stoichiometrically to monomeric G-actin and actin protomers. Cofilin intercalates between longitudinally associated actin monomers within the filament. Under specific conditions, It cleaves the filaments and accelerates actin subunits dissociation from their ‘pointed′ ends. It is essential for viability and vital for many cellular processes involving actin remodeling, such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis etc. Cofilin activity is regulated through reversible phosphorylation and dephosphorylation. In phosphorylated form, it is inactive and unable to bond with actin. Phosphorylation of cofilin is regulated by at least four protein kinases: LIM Kinase-1, LIM Kinase-2, Testicular Kinase-1, and Testicular Kinase-2. S-nitrosylation of cofilin is involved in vascular endothelial growth factor (VEGF)-stimulated endothelial cytoskeleton remodeling and migration. Over-expression of cofilin suppresses growth and invasion of non-small cell lung cancer. Dysregulation of cofilin is associated with neurodegeneration.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For prolonged storage, freeze in working aliquots at -20 ℃. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD03791485
NACRES NA.46

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 IgG fraction of antiserum
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen ~19 kDa
species reactivity canine, rat, human, mouse
technique(s) indirect immunofluorescence: 1:1,000 using using mouse NIH/3T3 fibroblasts
microarray: suitable
western blot: 1:10,000 using using whole extracts of human A-431, rat PC-12, mouse NIH/3T3, and dog MDCK kidney cells
UniProt登记号 P23528
运输 dry ice
储存温度 ?20℃
Gene Information human ... CFL1(1072), CFL2(1073)
mouse ... Cfl1(12631), Cfl2(12632)
rat ... Cfl1(29271), Cfl2(366624)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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