半岛bd体育手机客户端 说明
一般描述
The antibody specifically recognizes α and γ smooth muscle actin.
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. Its expression is related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).
Monoclonal Anti-Actin, Smooth Muscle, reacts with human, monkey, chicken, rat, and mouse smooth muscle actin.
Monoclonal Anti-Actin, Smooth Muscle (mouse IgG2b isotype) is derived from the hybridoma CGA7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified chicken gizzard actin. α-actin 2 (ACTA2) codes for a smooth muscle specific α-actin.
免疫原
partially purified chicken gizzard actin.
应用
Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse has been used in:
生化/生理作用
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Mutations in alpha-actin 2 (ACTA2) results in thoracic aortic aneurysm, Moyamoya disease, coronary artery disease, stroke and multisystemic smooth muscle dysfunction syndrome. It plays a functional role in the smooth muscle of non-vascular tissues.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00162107 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | CGA7, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~42 kDa |
| species reactivity | chicken, mouse, rat, human, monkey |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunocytochemistry: suitable immunohistochemistry indirect ELISA: suitable western blot: 5-10 μg/mL using chicken gizzard total extract |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | P62736 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | chicken ... ACTA2(423787) human ... ACTA2(59) mouse ... Acta2(11475) rat ... Acta2(81633) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
