PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb

半岛bd体育手机客户端 说明

一般描述

This PhosphoDetect Anti-Chk1 (pSer???) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer???).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser³¹⁷.
Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser³¹⁷in UV-treated COS cells.

免疫原

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser??? phosphorylation site of human Chk1

包装

50 μL in Plastic ampoule

应用

Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM HEPES, 100 ?g/ml BSA, 50% glycerol, pH 7.5.

重悬

Do not aliquot.

分析说明

Positive Control
Lysates from COS cells treated with UV

其他说明

Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Zhao, H. and Piwnica-Worms, H. 2001.Mol. Cell. Biol.21,4129.
Shieh, S.Y., et al. 2000.Genes Dev.14,289.
Martinho, R.G., et al. 1998.EMBO J.17,7239.
Zeng, Y., et al. 1998.Nature395,507.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	liquid
不包含	preservative
species reactivity	mouse, human, rat, monkey
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1