半岛bd体育手机客户端 说明
一般描述
Stromal interaction molecule 1 (STIM1) is a conserved single-pass transmembrane protein. STIM1 is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 localizes predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca2+levels.
免疫原
synthetic peptide corresponding to amino acids 657-683 of human STIM1, conjugated to KLH via an N-terminal residue. The corresponding sequence is identical in rat, mouse, bovine, monkey, and dog.
应用
Anti-STIM1 (C-terminal) antibody produced in rabbit has been used in:
生化/生理作用
Stromal interaction molecule 1 (STIM1) is required for the activation of store-operated Ca2+influx. STIM1 undergoes rapid oligomerization and redistributes into discrete spots (punctae) that move towards and accumulate in the cell periphery, possibly to activate Orai1 that is located in the plasma membrane. Overexpression of STIM1 and Orai1 together markedly increases the calcium release-activated channels (CRAC) current (I-CRAC). Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex plays a major role in the activation of store-operated calcium (SOC) channel in response to internal Ca2+store depletion.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 200 |
偶联物 | unconjugated |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous solution |
分子量 | antigen 90 kDa |
species reactivity | rat, mouse, human |
浓度 | ~1 mg/mL |
technique(s) | immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells. indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells. western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells. |
UniProt登记号 | Q13586 |
运输 | dry ice |
储存温度 | ?20℃ |
Gene Information | human ... STIM1(6786) mouse ... Stim1(20866) rat ... Stim1(117086) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich