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Anti-EEA1 antibody, Mouse monoclonal

Anti -EEA1 抗体,小鼠单克隆

品牌
Sigma-Aldrich
货号
E7659
规格纯度
clone EEA1-N19, purified from hybridoma cell culture
价格
6732.28 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-EEA1 antibody, Mouse monoclonal
Anti-MSTP105
Anti-MST105
Anti-ZFYVE2
Monoclonal Anti-Early endosomal antigen 1
Anti -EEA1 抗体,小鼠单克隆
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-EEA1 (mouse IgG2a isotype) is derived from the hybridoma EEA1-N19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EEA1. Early Endosome Antigen 1 (EEA1) is a dimer. It comprises extensive coiled-coil regions, and at its C-terminus contains a cysteine-rich zinc-finger-like domain named FYVE domain.
The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic face of early endosomes. EEA1 is a autoantigen associated with subacute cutaneous systemic lupus erythematosus and is a coiled-coil protein localized to early endosomes and cytosol.

应用

Anti-EEA1 antibody, Mouse monoclonalhas been used in:

  • immunoblotting
  • immunoprecipitation
  • cell fractionation
  • indirect immunofluorescence
  • immunohistochemistry


Monoclonal Anti-EEA1 antibody produced in mouse is suitable for the following applications:
  • Immunoprecipitation
  • Indirect immunofluorescence
  • Western blotting at a concentration of 1-2μg/mL using whole extract of rat NRK cells

生化/生理作用

Early Endosome Antigen 1 (EEA1) FYVE domain is involved in intracellular trafficking and is implicated in the specific localization of EEA1 to endosomes . Endosomal targeting of EEA1 also requires its binding to the active form of the small GTPase Rabaptin-5 (Rab5). The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns 3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. Monoclonal Anti-EEA1 may be used as an early endosome marker.
Early Endosome Antigen 1 (EEA1) controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population. Phosphatidylinositol 3-phosphate (PtdIns(3)P) is essential for the localization and function of EEA1.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 unconjugated
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 EEA1-N19, monoclonal
形式 buffered aqueous solution
分子量 antigen ~160 kDa
species reactivity rat, mouse, human
浓度 ~1.0 mg/mL
technique(s) immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using whole extract of rat NRK cells
同位素/亚型 IgG2a
UniProt登记号 Q15075
运输 dry ice
储存温度 ?20℃
Gene Information human ... EEA1(8411)
mouse ... Eea1(216238)
rat ... Eea1(314764)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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