Anti-Transcription factor EB Antibody

半岛bd体育手机客户端 说明

一般描述

The protein named Transcription factor EB (TFEB) or alternatively in older literature, Class E basic helix-loop-helix protein 35 (bHLHe35) and encoded by the human gene TFEB/BHLH35 is a transcription factor that specifically recognizes and binds E-box sequences (5′-CANNTG-3′) on DNA. Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. TFEB in association with the protein TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also plays a central role in expression of lysosomal genes. TFEB specifically recognizes and binds the CLEAR-box sequence (5′-GTCACGTGAC-3′) present in the regulatory region of many lysosomal genes and through binding acts as a positive regulator of their expression. Finally, TFEB appears to be a positive regulator for autophagy related gene expression, heavy chain immunoglobulin gene expression, and genes necessary for the proper vascularization of important tissues such as the placenta.

免疫原

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human Transcription factor EB.

应用

Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Detect TFEB using this rabbit polyclonal antibody, Anti-Transcription factor EB Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: 10 ?g from a representative lot immunoprecipitated Transcription factor EB in 500 ?g of Raji cell lysate. Immunopecipitated Transcription factor EB was then detected in Western Blot.

质量

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 ?g/mL of this antibody detected Transcription factor EB in 10 ?g of Raji cell lysate.

目标描述

~58 kDa and ~40 kDa observed. Uniprot describes two isoforms at 44 kDa and 53 kDa This protein has been observed at 44-53 kDa due to sumoylation (Miller, A. J., et al. (2005). J Biol Chem. 280(1):146-55.).

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	chimpanzee (based on 100% sequence homology), baboon (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), gibbon (based on 100% sequence homology), orangutan (based on 100% sequence homology)
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_009093
UniProt登记号	P19484
运输	wet ice
Gene Information	human ... TFEB(7942)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable