Anti-phospho-CYLD Antibody (Ser418)

半岛bd体育手机客户端 说明

一般描述

CYLD is a deubiquitinating enzyme that inactivates transcription factor NF-kappaB using TNFRs and is essential for normal cellular homeostasis of skin appendages. Deactivation or mutation of CYLD causes a loss of the deubiquitination activity and increases tumorigenesis. Loss of CYLD specifically results in familial cylindromatosis which is characterized by a predisposition to cylindromas, tumors of skin appendages. CYLD has been shown to cleave the lysine 63-linked polyubiquitin chain from proteins and the inactivity of CYLD has also been noticed in many other human cancers.

特异性

This antibody detects phospho-CYLD at Ser418. The phosphorylation site of CYLD shares some sequence homology with serine phosphorylation site of c-Cbl.

免疫原

Linear peptide corresponding to human phospho-CYLD (Ser418).

应用

Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-CYLD (Ser418) in 10 ?g of human spleen and Jurkat lysate.
Western Blotting Analysis: A representative lot detected phospho-CYLD (Ser418) in PMA and Ionomycin-treated Jurkat cell lysate (Reiley, W., et al. (2005). Mole. Cell Biol. 25(10):3886-3895).
This Anti-phospho-CYLD Antibody (Ser418) is validated for use in Western Blotting for the detection of phospho-CYLD (Ser418).

质量

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-CYLD (Ser418) in 10 ?g of Raji cell lysate.

目标描述

~110 kDa observed.
Uncharacterized band(s) may appear in some lysates.

外形

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human
technique(s)	western blot: suitable
NCBI登记号	NP_001035814
UniProt登记号	Q9NQC7
运输	wet ice
Gene Information	human ... CYLD(1540)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1