Anti-YTHDF2 Antibody

半岛bd体育手机客户端 说明

一般描述

YTH domain-containing family protein 2 (YTHDF2), also known as CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2, is also encoded by the gene name YTHDF2 and HGRG8. YTHDF2 recognizes specifically and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs. Recent studies have shown that YTHDF2 may play an important role in the efficiency of mRNA splicing, processing and stability. Additionally, YTHDF2 has been shown to act as a regulator of mRNA stability. And in the process of binding to m6A-containing mRNAs, YTHDF2 has been implicated to localize to mRNA decay sites, such as processing bodies (P-bodies), which leads to mRNA degradation.

免疫原

Epitope: Localization to mRNA processing bodies (P-bodies)
KLH-conjugated linear peptide corresponding to the localization of mRNA processing bodies (P-bodies) to human YTHDF2.

应用

Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
This Anti-YTHDF2 antibody is validated for use in WB, IC for the detection of YTHDF2.
Western Blotting Analysis: 15 ?g/mL from a representative lot detected YTHDF2 in WT HeLa cell lysate and YTHDF2 overexpressing lysate (Chuan He, Univerity of Chicago).
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected YTHDF2 in HeLa cells (Chuan He, Univerity of Chicago).

质量

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected YTHDF2 in 10 ?g of C6 cell lysate.

目标描述

~62 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, rat
technique(s)	immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_001166599
UniProt登记号	Q9Y5A9
运输	wet ice
Gene Information	human ... YTHDF2(51441)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable