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位置: 首页> 品牌> Sigma-Aldrich> Anti-phospho TIE2/TEK Antibody (Tyr992)

Anti-phospho TIE2/TEK Antibody (Tyr992)

品牌
Sigma-Aldrich
货号
ABF131
规格纯度
0.25 mg/mL, from rabbit
价格
6022.61 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-phospho TIE2/TEK Antibody (Tyr992)
Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, hTIE2, p140 TEK, CD202b
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

TIE2/TEK (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2/TEK are associated with inherited venous malformations; the TIE2/TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

免疫原

KLH-conjugated linear peptide corresponding to the Protein Kinase Domain of human TIE2/TEK phosphorylated at Tyr992.
Epitope: Protein Kinase Domain

应用

Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992).
Immunoprecipitation Analysis: 5 ?L from a representative lot immunoprecipitated phospho TIE2/TEK (Tyr992) from Pervanadate treated HUVEC cell lysate. Immunoprecipated sample was then subjected to Western Blotting using a 1:5,000 dilution of this antibody.

Peptide Inhibition Assay Analysis: A 1:500 dilution from a representative lot detected phospho TIE2/TEK (Tyr992) from transfected HEK293T lysates when pre-incubated without or with non-phospho peptide, and not when pre-incubated with phospho peptide.
Research Category
Inflammation & Immunology

质量

Evaluated by Western Blotting in untreated and Pervanadate treated HUVEC cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho TIE2/TEK (Tyr992) in 10 ?g of Pervanadate treated HUVEC cell lysate, and not in untreated HUVEC cell lysate.

目标描述

~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

联系

Replaces: 09-407

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
species reactivity (predicted by homology) zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
浓度 0.25 mg/mL
technique(s) immunoprecipitation (IP): suitable
inhibition assay: suitable (peptide)
western blot: suitable
NCBI登记号 NP_000450
UniProt登记号 Q02763
运输 wet ice
Gene Information human ... TEK(7010)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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